The Little Red Train is a good friend of Annie, and despite his small size, he is very determined and very strong. He appeared in the episodes "Go West, Young Train" and "Annie, Get Your Microphone!". Certain season 2 episodes and special episodes in Season 1 (such as " The Christmas Wish") have better-quality animation compared with most season 1 episodes.

DisneyNOW had both seasons of the show but was removed once in April 2019, then twice in November 2019 to move the series over to Disney+.The show is also designed to encourage viewer interaction (such as encouraging the audience to pat their knees, gesture or sing along to help the characters succeed on their "mission").

The theme song now has the characters clap before saying "Little Einsteins." Quincy also now uses a drum in the final scene of the song.Big Jet is the main antagonist and a common villain for the Little Einsteins. He often interrupts the group's missions, loves to steal everything to keep for himself, cheats at every game he participates in, and loathes losing. However, in " Show and Tell," after stealing all of the group's possessions for their school's show and tell, Big Jet befriends the team and never attempted to rival them again after this episode (mainly because that episode was his final appearance). The main theme seamlessly transitions into the episode's title card, which is set on a stage. The Little Einsteins shush each other and get ready, then either Annie or Quincy would reveal the name of the episode, June introduces the main artwork that appears throughout the episode, and Leo introduces the main song that would be showcased throughout the events of the episode, all (besides most artwork) using homemade signage. Little Einsteins in America, UK, Australia, Bahasa Indonesia, Italy, Spain, Philippines, and Portugal. [2] [3] [4] Multiple types of merchandise were made in the series of successful years including toys, clothes, DVDs, CDs, books, video games, bed material, cereal, and more. Most of the merchandise have been discontinued shortly after the series ended. In the UK, some of the dialogue has been changed to fit the British culture, like "Yeah!" being changed to "Yes!". The show has been dubbed into other languages as well: